LYN Polyklonaler Antikörper

LYN Polyklonal Antikörper für FC, IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 18135-1-AP

Synonyme

JTK8, LYN, Tyrosine protein kinase Lyn

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 18135-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 18135-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 18135-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18135-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18135-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 18135-1-AP

  IP Result of anti-LYN (IP:18135-1-AP, 4ug; Detection:18135-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human kidney using 18135-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 18135-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 18135-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 18135-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 18135-1-AP

  Immunofluorescent analysis of HeLa cells using 18135-1-AP (LYN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 18135-1-AP

  1X10^6 Jurkat cells were stained with 0.2ug LYN antibody (18135-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, L02-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

18135-1-AP bindet in WB, IP, IHC, IF, FC, ELISA LYN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LYN fusion protein Ag12723
• Vollständiger Name: v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• Berechnetes Molekulargewicht: 512 aa, 59 kDa
• Beobachtetes Molekulargewicht: 53-56 kDa, 66-70 kDa
• GenBank-Zugangsnummer: BC075002
• Gene symbol: LYN
• Gene ID (NCBI): 4067
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.

Publikationen

Species	Application	Title
human	IHC	Front Cell Dev Biol; Immune Characteristics of LYN in Tumor Microenvironment of Gliomas.; Authors - Chonghua Jiang