Perilipin 5 Polyklonaler Antikörper

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• KD/KO Validated

Perilipin 5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 26951-1-AP

Synonyme

LSDP5, OXPAT, Perilipin 5, PLIN5

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Galerie der Validierungsdaten

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  WB analysis using 26951-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 26951-1-AP

  WB result of Perilipin 5 antibody (26951-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Perilipin 5 transfected MCF-7 cells.

• 

  WB analysis of pig heart using 26951-1-AP

  pig heart tissue were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 26951-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brown adipose using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, Hausschwein-Herzgewebe, Mausherzgewebe, Mauslebergewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, braunes Mausfettgewebe, humanes Herzgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

26951-1-AP bindet in WB, IP, IHC, IF, ELISA Perilipin 5 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Perilipin 5 fusion protein Ag25272
• Vollständiger Name: lipid storage droplet protein 5
• Berechnetes Molekulargewicht: 463 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51-55 kDa
• GenBank-Zugangsnummer: BC131524
• Gene symbol: LSDP5
• Gene ID (NCBI): 440503
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Perilipin 5 (also known as LSDP5/OXPAT) is a lipid droplet (LD) coat protein that promotes association of LDs with mitochondria and is mainly present in tissues with a high fat-oxidative capacity, such as heart, skeletal muscles and brown adipose tissue. It plays an important role in the regulation of cardiac lipid storage and function.

Publikationen

Species	Application	Title
mouse	WB	J Mol Cell Biol; Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice; Authors - Xinyu Bao
mouse	WB	EMBO Rep; miR-183 and miR-96 orchestrate both glucose and fat utilization in skeletal muscle.; Authors - Hui Wang
mouse	WB	Int J Mol Sci; Effects of Simvastatin on Lipid Metabolism in Wild-Type Mice and Mice with Muscle PGC-1α Overexpression.; Authors - Miljenko V Panajatovic
mouse	WB	Int Immunopharmacol; Perilipin 5 protects against oxygen-glucose deprivation/reoxygenation-elicited neuronal damage by inhibiting oxidative stress and inflammatory injury via the Akt-GSK-3β-Nrf2 pathway.; Authors - Kang Huo
human	WB,IF	Hum Cell; Aryl hydrocarbon receptor promotes lipid droplet biogenesis and metabolic shift in respiratory Club cells.; Authors - Hsueh-Chun Wang
mouse	WB	Biochem Biophys Res Commun; Perilipin 5 ameliorates high-glucose-induced podocyte injury via Akt/GSK-3β/Nrf2-mediated suppression of apoptosis, oxidative stress, and inflammation.; Authors - Jie Feng