LRCH3 Polyklonaler Antikörper

LRCH3 Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15800-1-AP

Synonyme

LRCH3

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Galerie der Validierungsdaten

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  WB analysis of human liver using 15800-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15800-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 15800-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 15800-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15800-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15800-1-AP

  IP Result of anti-LRCH3 (IP:15800-1-AP, 3ug; Detection:15800-1-AP 1:1000) with mouse liver tissue lysate 12500ug.

• 

  IF Staining of A431 using 15800-1-AP

  Immunofluorescent analysis of A431 cells using 15800-1-AP (LRCH3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, humanes Herzgewebe, humanes Milzgewebe, Jurkat-Zellen, Mauslungengewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15800-1-AP bindet in WB, IP, IF,ELISA LRCH3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LRCH3 fusion protein Ag8511
• Vollständiger Name: leucine-rich repeats and calponin homology (CH) domain containing 3
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC007504
• Gene symbol: LRCH3
• Gene ID (NCBI): 84859
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.