LPCAT3 Monoklonaler Antikörper

LPCAT3 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1C1G3

Kat-Nr. : 67882-1-Ig

Synonyme

C3F, LPCAT, LPCAT3, LPLAT 5, LPSAT, Lyso PC acyltransferase, Lyso PC acyltransferase 3, Lyso PS acyltransferase, MBOAT5, nessy, OACT5

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Galerie der Validierungsdaten

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  WB analysis of mouse stomach using 67882-1-Ig

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 67882-1-Ig (LPCAT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig stomach using 67882-1-Ig

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 67882-1-Ig (LPCAT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 67882-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 67882-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 67882-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 67882-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Magengewebe, Haussschwein-Magengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

67882-1-Ig bindet in WB, IHC, ELISA LPCAT3 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LPCAT3 fusion protein Ag6472
• Vollständiger Name: lysophosphatidylcholine acyltransferase 3
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC065194
• Gene symbol: LPCAT3
• Gene ID (NCBI): 10162
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lysophosphatidylcholine acyltransferases (LPCATs) are among the lysophopholipid acyltransferases (LPLATs) that play an important role in lipid metabolism and homeostasis by regulating the abundance of different phosphatidylcholine (PC) species. Lysophosphatidylcholine acyltransferase 3 (LPCAT3) is a member of the LPCAT family that primarily regulates the levels of arachidonic PC species. LPCAT3 is abundantly expressed in the testes, kidneys and metabolic tissues, including liver, intestine and adipose tissues. LPCAT3 is involved in the occurrence and development of atherosclerosis, intestinal tumors, and nonalcoholic steatohepatitis (NASH)(PMID: 35711841).

Publikationen

Species	Application	Title
human	WB	J Exp Clin Cancer Res; Lipid reprogramming induced by the TFEB-ERRα axis enhanced membrane fluidity to promote EC progression.; Authors - Xiaodan Mao
human	WB	Ecotoxicol Environ Saf; Cadmium perturbed lipid profile and induced liver dysfunction in mice through phosphatidylcholine remodeling and promoting arachidonic acid synthesis and metabolism; Authors - Jie Gu
rat	WB	Sci Total Environ; Insight into the effect of a heavy metal mixture on neurological damage in rats through combined serum metabolomic and brain proteomic analyses; Authors - Jie Xie
human	WB	Redox Biol; Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis; Authors - Xue Yang