LPCAT2 Polyklonaler Antikörper

LPCAT2 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15082-1-AP

Synonyme

Acyltransferase like 1, AYTL1, LPC acyltransferase 2, LPCAT 2, LPCAT2, Lyso PAF acetyltransferase, LysoPAFAT, LysoPC acyltransferase 2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, RAW 264.7-Zellen
Erfolgreiche Detektion in IFA431-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunfluoreszenz (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

15082-1-AP bindet in WB, IF, ELISA LPCAT2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen LPCAT2 fusion protein Ag7156
Vollständiger Name lysophosphatidylcholine acyltransferase 2
Berechnetes Molekulargewicht 60 kDa
Beobachtetes Molekulargewicht 53 kDa
GenBank-ZugangsnummerBC002472
Gene symbol LPCAT2
Gene ID (NCBI) 54947
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
WB protocol for LPCAT2 antibody 15082-1-APProtokoll herunterladen
IF protocol for LPCAT2 antibody 15082-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Antioxidants (Basel)

Mitochondrial Reactive Oxygen Species Formation Determines ACSL4/LPCAT2-Mediated Ferroptosis

Authors - Melanie Merkel

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Melanie (Verified Customer) (04-22-2022)

LPCAT2 antibody was diluted 1:1500 in 5% milk in TBST and applied to protein samples from Hek293T cells. The bands were very clear. The antibody could have been diluted even more.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1500
  • Cell Tissue Type: Hek293T cells
FH

Christian (Verified Customer) (12-17-2018)

10 μg protein per lane run on 10% Tris-Glycine gel. Primary incubation 1:400 overnight @ 4°C. Secondary antibody and biotinylated marker were supplied from a different vendor and used and used at optimized dilutions.LPCAT2 siRNA was used to check antibody specificity.The antibody performed excellently, will try at a dilution of 1:800 next time.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: RAW264.7
LPCAT2 Antibody Western Blot, validation (1:400 dilution) in RAW264.7 (Cat no:15082-1-AP)