LPCAT1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

LPCAT1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

8B6E9

Kat-Nr. : 66044-1-Ig

Synonyme

Acyltransferase like 2, AYTL2, LPC acyltransferase 1, lpcat, LPCAT 1, LPCAT1, Lyso PAF acetyltransferase, LysoPAFAT, LysoPC acyltransferase 1, PFAAP3

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Galerie der Validierungsdaten

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  WB analysis using 66044-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of MCF-7 using 66044-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66044-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66044-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 66044-1-Ig

  PC-12, C6, Raw264.7, NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse lung using 66044-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using LPCAT1 antibody (66044-1-Ig, Clone: 8B6E9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), SFTPC antibody (10774-1-AP, green).

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  IF Staining of HeLa using 66044-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LPCAT1 antibody (66044-1-Ig, Clone: 8B6E9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mauslungengewebe, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

66044-1-Ig bindet in WB, IHC, IF, ELISA LPCAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LPCAT1 fusion protein Ag9060
• Vollständiger Name: lysophosphatidylcholine acyltransferase 1
• Berechnetes Molekulargewicht: 534 aa, 59 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC020166
• Gene symbol: LPCAT1
• Gene ID (NCBI): 79888
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LPCAT1, also named as AYTL2, PFAAP3 and LysoPAFAT, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. The expression level of LPCAT1 is able to differentiate prostate cancer from noncancerous prostatic changes, and correlates to the tumor grade of prostate cancer. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC).

Publikationen

Species	Application	Title
human	WB	Cell Metab; Oncogene Amplification in Growth Factor Signaling Pathways Renders Cancers Dependent on Membrane Lipid Remodeling.; Authors - Junfeng Bi ; KD Validated
mouse,rat	WB	Biochim Biophys Acta Mol Basis Dis; Stearoyl-CoA desaturase 1 deficiency exacerbates palmitate-induced lipotoxicity by the formation of small lipid droplets in pancreatic β-cells; Authors - Justyna Janikiewicz
mouse, human	IHC, WB	Front Oncol; Gut Microbiota Dysbiosis Accelerates Prostate Cancer Progression Through Increased LPCAT1 Expression and Enhanced DNA Repair Pathways.; Authors - Yufei Liu
mouse	IHC	Sci Rep; No evidence for carcinogenicity of titanium dioxide nanoparticles in 26-week inhalation study in rasH2 mouse model; Authors - Shotaro Yamano
human	WB	Biochem Biophys Res Commun; A miR-205-LPCAT1 axis contributes to proliferation and progression in multiple cancers.; Authors - Fang Liu ; KD Validated
human	WB,IF,IHC	Cell Death Dis; LPCAT1 reprogramming cholesterol metabolism promotes the progression of esophageal squamous cell carcinoma.; Authors - Mingyue Tao ; KD Validated