LONP1 Monoklonaler Antikörper

LONP1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

1C6C12

Kat-Nr. : 66043-1-Ig

Synonyme

hLON, LON, lon peptidase 1, mitochondrial, Lon protease like protein, LonHS, LONP, LONP1, PIM1, PRSS15, Serine protease 15

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 66043-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66043-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 66043-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66043-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66043-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66043-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66043-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66043-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66043-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66043-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66043-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human lung cancer using 66043-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 40x lens).

• 

  IHC staining of human heart using 66043-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human heart using 66043-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 40x lens).

• 

  IHC staining of human liver using 66043-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human liver using 66043-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 40x lens).

• 

  IF Staining of C6 using 66043-1-Ig

  Immunofluorescent analysis of C6 cells using 66043-1-Ig (LONP1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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  IF Staining of C6 using 66043-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using LONP1 antibody (66043-1-Ig, Clone: 1C6C12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidine (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ROS1728-Zellen, HEK-293-Zellen, humanes Herzgewebe, humanes Lebergewebe, Jurkat-Zellen, L02-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Herzgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: C6-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66043-1-Ig bindet in WB, IHC, IF, CoIP, ELISA LONP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LONP1 fusion protein Ag7306
• Vollständiger Name: lon peptidase 1, mitochondrial
• Berechnetes Molekulargewicht: 106 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC000235
• Gene symbol: LONP1
• Gene ID (NCBI): 9361
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).

Publikationen

Species	Application	Title
mouse,human	CoIP	Acta Pharm Sin B; Miriplatin-loaded liposome, as a novel mitophagy inducer, suppresses pancreatic cancer proliferation through blocking POLG and TFAM-mediated mtDNA replication; Authors - Xiaowei Wang
human	WB	EMBO J; Stress-dependent macromolecular crowding in the mitochondrial matrix; Authors - Elianne P Bulthuis
human,mouse	IHC,WB	Mol Med; ATF5 regulates tubulointerstitial injury in diabetic kidney disease via mitochondrial unfolded protein response; Authors - Yifei Liu
human	WB	Mol Cell; A mitochondrial iron-responsive pathway regulated by DELE1; Authors - Yusuke Sekine ; KD Validated
human,mouse	WB	BMC Neurosci; Exercise combined with postbiotics treatment results in synergistic improvement of mitochondrial function in the brain of male transgenic mice for Alzheimer's disease; Authors - Attila Kolonics