LNPEP Polyklonaler Antikörper

LNPEP Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55154-1-AP

Synonyme

CAP, Cystinyl aminopeptidase, IRAP, Leucyl cystinyl aminopeptidase, leucyl/cystinyl aminopeptidase, LNPEP, OTase, Oxytocinase, P LAP, PLAP, VP165

---

Galerie der Validierungsdaten

• 

  WB analysis of human brain using 55154-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 55154-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 55154-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55154-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55154-1-AP (LNPEP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55154-1-AP (LNPEP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, COLO 320-Zellen, humanes Herzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

55154-1-AP bindet in WB, IHC,ELISA LNPEP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: leucyl/cystinyl aminopeptidase
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 117-125 kDa
• GenBank-Zugangsnummer: NM_005575
• Gene symbol: LNPEP
• Gene ID (NCBI): 4012
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LNPEP, also named as OTASE, IRAP, P-LAP and Otase, may be involved in the inactivation of neuronal peptides in the brain. LNPEP cleaves Met-enkephalin and dynorphin. It binds angiotensin IV and may be the angiotensin IV receptor in the brain. It can be detected a protein with molecular mass of 165 kDa in several human tissues including heart, small intestine, kidney and 140 kDa in brain. LNPEP contains 18 potential N-glycosylation sites so that the differential usage of the glycosylation site in the brain causes the difference in the molecular mass of the enzyme.(PMID:11389728). Two forms of LNPEP are purified from human placenta: one is a monomeric, 61-kDa protein and the other is a heterodimeric protein with 64- and 97-kDa subunits(PMID:10601849). It can form a homodimer of 280 kDa(PMID:10601849). This antibody is specific to LNPEP.