LIS1 Polyklonaler Antikörper

LIS1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 20678-1-AP

Synonyme

LIS 1, LIS1, LIS2, Lissencephaly 1 protein, MDCR, MDS, PAF AH 45 kDa subunit, PAF AH alpha, PAFAH, PAFAH alpha, PAFAH1B1, PAFAHA

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Galerie der Validierungsdaten

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  WB analysis using 20678-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20678-1-AP (LIS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20678-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20678-1-AP (LIS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20678-1-AP (LIS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20678-1-AP (LIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20678-1-AP bindet in WB, IHC, ELISA LIS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: NM_000430
• Gene symbol: PAFAH1B1
• Gene ID (NCBI): 5048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet-activating factor acetylhydrolase 1B subunit alpha (LIS1), a critical mediator of neuronal migration in developing brain, is expressed throughout life. LIS1 forms a homodimer that interacts directly with the dynein complex and is essential for its enrichment at dynamic microtubule plus ends in mammalian cells. In addition to regulating the association of dynein with microtubule plus ends, LIS1 is required for efficient transport of many dynein-associated cargos and proper localization of Golgi complexes, endosomes, lysosomes, and mitochondria. The LIS1 protein has a calculated molecular mass of ~45 kDa.(PMID: 29150182, PMID: 29562183, PMID: 15800059)

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Aurélie (Verified Customer) (06-13-2019)

Good antibody in WB with quite low background, 1/2000 could be enough

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse Cortex

LIS1 Antibody Western Blot, validation (1/1000 dilution) in Mouse Cortex (Cat no:20678-1-AP)