LIPC Polyklonaler Antikörper

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• KD/KO Validated

LIPC Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 21133-1-AP

Synonyme

HDLCQ12, Hepatic lipase, Hepatic triacylglycerol lipase, HL, HTGL, Lipase member C, lipase, hepatic, LIPC, LIPH

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 21133-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21133-1-AP (LIPC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 21133-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21133-1-AP (LIPC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 21133-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21133-1-AP (LIPC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 21133-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21133-1-AP (LIPC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 21133-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21133-1-AP (LIPC Antibody) at dilution of 1:50.

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  IHC staining of human lung cancer using 21133-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21133-1-AP (LIPC Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 21133-1-AP

  Immunofluorescent analysis of HepG2 cells using 21133-1-AP (LIPC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HepG2-Zellen, Mauslebergewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 4 publications below

Produktinformation

21133-1-AP bindet in WB, IHC, IF, ELISA LIPC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIPC fusion protein Ag15538
• Vollständiger Name: lipase, hepatic
• Berechnetes Molekulargewicht: 499 aa, 56 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC146659
• Gene symbol: LIPC
• Gene ID (NCBI): 3990
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIPC, also named as HTGL and HL, belongs to the AB hydrolase superfamily and Lipase family. It has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. LIPC is an important enzyme in HDL metabolism. It binds heparin.

Publikationen

Species	Application	Title
human	IHC	Cell Res; Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.; Authors - Shuaishuai Teng
human,mouse	WB,IHC	J Hematol Oncol; High-resolution Hi-C maps highlight multiscale 3D epigenome reprogramming during pancreatic cancer metastasis.; Authors - Bo Ren ; KO Validated
mouse	IHC	Lab Invest; Cholestasis impairs hepatic lipid storage via AMPK and CREB signaling in hepatitis B virus surface protein transgenic mice.; Authors - Karuna Irungbam
human	IHC	J Transl Med; Downregulation of TUSC3 promotes EMT and hepatocellular carcinoma progression through LIPC/AKT axis; Authors - Ruxia Deng ; KD Validated
rat	WB	Arch Pharm Res; Protective effects of L-theanine on rats with dextran sulfate sodium-induced inflammatory bowel disease.; Authors - Ling Chen