LINGO1 Polyklonaler Antikörper

LINGO1 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19097-1-AP

Synonyme

LERN1, LINGO1, LRRN6A, UNQ201

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 19097-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19097-1-AP (LINGO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19097-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19097-1-AP (LINGO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 19097-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 19097-1-AP (LINGO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of SH-SY5Y using 19097-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug LINGO1 antibody (19097-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19097-1-AP bindet in WB, FC, ELISA LINGO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LINGO1 fusion protein Ag13553
• Vollständiger Name: leucine rich repeat and Ig domain containing 1
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC011057
• Gene symbol: LINGO1
• Gene ID (NCBI): 84894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LINGO-1 is a transmembrane receptor that contains 12 leucine-rich repeats and an immunoglobulin domain. It is expressed primarily in the central nervous system. LINGO-1 binds Nogo-66 receptor (NgR) and p75 and that is a functional component of the NgR/p75 signaling complex (PMID: 14966521). This protein has a calculated molecular weight of 70 kDa and migrates at higher molecular weight due to glycosylation (PMID: 21872576; 25257685; 18326721).