LIN28B Polyklonaler Antikörper

LIN28B Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, PLA, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 24017-1-AP

Synonyme

CSDD2, lin 28 homolog B (C. elegans), Lin 28B, LIN28B, Protein lin 28 homolog B

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Galerie der Validierungsdaten

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  WB analysis using 24017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24017-1-AP (LIN28B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24017-1-AP

  IP Result of anti-LIN28B (IP:24017-1-AP, 4ug; Detection:24017-1-AP 1:500) with HEK-293 cells lysate 1480ug.

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  IF Staining of NCCIT using 24017-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using LIN28B antibody (24017-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF: NCCIT-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

24017-1-AP bindet in WB, PLA, IP, IHC, IF, ELISA LIN28B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIN28B fusion protein Ag21241
• Vollständiger Name: lin-28 homolog B (C. elegans)
• Berechnetes Molekulargewicht: 250 aa, 27 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC137526
• Gene symbol: LIN28B
• Gene ID (NCBI): 389421
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIN28B, also named as CSDD2, belongs to the lin-28 family. It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7). LIN2B is specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells. However, it is suppressed in most adult tissues except for skeletal and cardiac muscle. The calculated molecular weight of LIN28B is 27 kDa, but a 35 kDa band has been detected in some articles (PMID: 33029224, 33378003).

Publikationen

Species	Application	Title
mouse	IF	Front Immunol; Lin28 regulates thymic growth and involution and correlates with MHCII expression in thymic epithelial cells; Authors - Shiyun Xiao
human	WB,PLA	iScience; Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells; Authors - Nam-Kyung Yu
mouse	WB	Pharm Biol; Peptides from the croceine croaker (Larimichthys crocea) swim bladder attenuate busulfan-induced oligoasthenospermia in mice.; Authors - Xi Zhao
human	IP, IHC	Int J Clin Exp Pathol; Lin28B is a novel prognostic marker in gastric adenocarcinoma.; Authors - Qian Hu