LIMS1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LIMS1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 20772-1-AP

Synonyme

LIMS1, PINCH, PINCH 1, PINCH1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 20772-1-AP

  WB result of LIMS1 antibody (20772-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMS1 transfected HeLa cells.

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  WB analysis of Caco-2 using 20772-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 20772-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 20772-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 20772-1-AP

  IP Result of anti-LIMS1 (IP:20772-1-AP, 4ug; Detection:20772-1-AP 1:500) with K-562 cells lysate 3600ug.

• 

  IHC staining of human oesophagus cancer using 20772-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 20772-1-AP (LIMS1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 20772-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 20772-1-AP (LIMS1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 20772-1-AP

  Immunofluorescent analysis of Hela cells, using LIMS1 antibody 20772-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 20772-1-AP

  1X10^6 HeLa cells were stained with 0.2ug LIMS1 antibody (20772-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HeLa-Zellen, humanes Nierengewebe, Mauslungengewebe
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Produktinformation

20772-1-AP bindet in WB, IP, IHC, IF, FC, ELISA LIMS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIMS1 fusion protein Ag14764
• Vollständiger Name: LIM and senescent cell antigen-like domains 1
• Berechnetes Molekulargewicht: 325 aa, 37 kDa
• Beobachtetes Molekulargewicht: 35-37 kDa
• GenBank-Zugangsnummer: BC005341
• Gene symbol: LIMS1
• Gene ID (NCBI): 3987
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Commun; PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.; Authors - Ling Guo ; KO Validated
human	WB,IP	J Cell Biol; A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.; Authors - Ling Guo ; KD Validated
human	WB	Theranostics; PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.; Authors - Xiaoxiao Wang
	WB	J Cell Sci; Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.; Authors - Clotilde Huet-Calderwood
human	WB	Amino Acids; PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.; Authors - Chunhong Cui ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yasmin (Verified Customer) (08-01-2019)

Excellent antibody for western blot. Works well at 1:1000 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000 for western blot
• Cell Tissue Type: HeLa, NIH3T3