LIFR Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LIFR Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Rezensionen (1)

Kat-Nr. : 22779-1-AP

Synonyme

CD118, LIF R, LIF receptor, LIFR, SJS2, STWS, SWS

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Galerie der Validierungsdaten

• 

  WB analysis of human skeletal muscle using 22779-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22779-1-AP (LIFR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22779-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22779-1-AP (LIFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 22779-1-AP

  IP Result of anti-LIFR (IP:22779-1-AP, 4ug; Detection:22779-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.

• 

  IHC staining of human skeletal muscle using 22779-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse cerebellum using 22779-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22779-1-AP (LIFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 22779-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22779-1-AP (LIFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 22779-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 22779-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 22779-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 22779-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 22779-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 22779-1-AP (LIFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 22779-1-AP

  1X10^6 HeLa cells were stained with .2ug LIFR antibody (22779-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

22779-1-AP bindet in WB, IP, IHC, IF, FC, ELISA LIFR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIFR fusion protein Ag18742
• Vollständiger Name: leukemia inhibitory factor receptor alpha
• Berechnetes Molekulargewicht: 1097 aa, 124 kDa
• Beobachtetes Molekulargewicht: 190 kDa
• GenBank-Zugangsnummer: BC153096
• Gene symbol: LIFR
• Gene ID (NCBI): 3977
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIFR, also known as CD118, is a subunit of a receptor for leukemia inhibitory factor (LIF). LIF is a pleiotropic cytokine of the interleukin-6 family which affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIFR is the low-affinity binding chain that, together with the high-affinity converter subunit gp130, forms a high-affinity receptor complex that mediates the action of LIF. The high-affinity complex also binds a related cytokine, oncostatin M (PMID: 8999038). LIFR has also been identified as a breast cancer metastasis suppressor that functions through the HIPPO-YAP pathway (PMID: 23001183).

Publikationen

Species	Application	Title
mouse		Nature; Exercise plasma boosts memory and dampens brain inflammation via clusterin.; Authors - Zurine De Miguel
mouse	WB	Nat Commun; A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis.; Authors - Fan Yao ; KO Validated
human	WB	Clin Transl Med; Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.; Authors - An-Dong Liu
human	WB,IHC,IF	Hum Mol Genet; Mutations in the leukemia inhibitory factor receptor (LIFR) gene and Lifr deficiency cause urinary tract malformations.; Authors - Anne Kosfeld
human	WB	J Exp Clin Cancer Res; miR-589 promotes gastric cancer aggressiveness by a LIFR-PI3K/AKT-c-Jun regulatory feedback loop.; Authors - Feifei Zhang
mouse	WB	Biotechnol Bioeng; Enzyme-free cell detachment mediated by resonance vibration with temperature modulation.; Authors - Yuta Kurashina

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Clarisse (Verified Customer) (12-12-2022)

Low signal, regardless of the method of fixation.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human cerebral organoids

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