LIF Polyklonaler Antikörper

LIF Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 26757-1-AP

Synonyme

CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 26757-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26757-1-AP (LIF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26757-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26757-1-AP (LIF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 26757-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP (LIF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26757-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP (LIF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 26757-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 26757-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse embryo using 26757-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using LIF antibody (26757-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, Mausembryogewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausembryogewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Produktinformation

26757-1-AP bindet in WB, IHC, IF, ELISA LIF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIF fusion protein Ag25076
• Vollständiger Name: leukemia inhibitory factor (cholinergic differentiation factor)
• Berechnetes Molekulargewicht: 202 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC093733
• Gene symbol: LIF
• Gene ID (NCBI): 3976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

Publikationen

Species	Application	Title
human	IF	Cell Stem Cell; LIF signaling regulates outer radial glial to interneuron fate during human cortical development; Authors - Madeline G Andrews
mouse	WB,IHC	Toxicology; Toxicity mechanism of peri-implantation pesticide beta-cypermethrin exposure on endometrial remodeling in early pregnant mice; Authors - Yong-Jiang Zhou
human	WB	Hum Reprod; The depot GnRH agonist protocol improves the live birth rate per fresh embryo transfer cycle, but not the cumulative live birth rate in normal responders: a randomized controlled trial and molecular mechanism study.; Authors - Bei Xu
human	IHC	Int J Cancer; Leukemia inhibitory factor is a novel biomarker to predict lymph node and distant metastasis in pancreatic cancer.; Authors - Wenna Jiang
human	WB	Reprod Biol Endocrinol; Expression and significance of miR-30d-5p and SOCS1 in patients with recurrent implantation failure during implantation window; Authors - Yuhao Zhao
mouse	IHC	Reproduction; Consistency and synchronization of AMPK-glycogen in endometrial epithelial cells are critical to the embryo implantation.; Authors - Li Nie