LGR6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LGR6 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 17658-1-AP

Synonyme

GPCR, LGR6, VTS20631

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Galerie der Validierungsdaten

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  WB analysis of HUVEC using 17658-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17658-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17658-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17658-1-AP (LGR6 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17658-1-AP (LGR6 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 17658-1-AP

  1X10^6 HeLa cells were stained with 0.2ug LGR6 antibody (17658-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, humanes Hirngewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Eierstockgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• ELISA: See 1 publications below

Produktinformation

17658-1-AP bindet in WB, IHC, FC, ELISA LGR6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LGR6 fusion protein Ag11909
• Vollständiger Name: leucine-rich repeat-containing G protein-coupled receptor 6
• Berechnetes Molekulargewicht: 915 aa, 99 kDa
• Beobachtetes Molekulargewicht: 104 kDa
• GenBank-Zugangsnummer: BC047905
• Gene symbol: LGR6
• Gene ID (NCBI): 59352
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LGR6 (Leucine-rich repeat-containing G-protein coupled receptor 6) is a seven-transmembrane protein. LGR6 is a high affinity receptor of R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. It potentially functions as a tumor suppressor. (PMID: 20223988; 20417836; 22615920)

Publikationen

Species	Application	Title
human		J Clin Invest; Maresin 1 activates LGR6 receptor promoting phagocyte immunoresolvent functions.; Authors - Nan Chiang ; KD Validated
human	WB	Mol Ther Oncolytics; Silencing LGR6 Attenuates Stemness and Chemoresistance via Inhibiting Wnt/β-Catenin Signaling in Ovarian Cancer.; Authors - Xiaohong Ruan
human	WB	PLoS One; Characterizing the cellular attachment receptor for Langat virus.; Authors - Raquel Rodrigues
mouse	WB	JBMR Plus; Evidence for Lgr6 as a Novel Marker of Osteoblastic Progenitors in Mice.; Authors - Vikram Khedgikar
mouse	ELISA	Proc Natl Acad Sci U S A; The Maresin 1-LGR6 axis decreases respiratory syncytial virus-induced lung inflammation; Authors - Nandini Krishnamoorthy
human	IHC	Aging (Albany NY); Comprehensive analysis of cuproptosis-related immune biomarker signature to enhance prognostic accuracy in gastric cancer; Authors - Jie Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marc (Verified Customer) (02-14-2023)

Used this antibody on immunofluorescence, but the expression of LGR6 in the spinal cord is low so we had to perform an immunofluorescence with tyramide amplification. With tyramide amplification the result is satisfactory.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Spinal Cord