LGR4 Polyklonaler Antikörper

LGR4 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 20150-1-AP

Synonyme

G protein coupled receptor 48, GPR48, LGR4

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Galerie der Validierungsdaten

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  IHC staining of human small intestine using 20150-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20150-1-AP (LGR4 antibody) at dilution of 1:2000 (under 10x lens).

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  IHC staining of human small intestine using 20150-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20150-1-AP (LGR4 antibody) at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human small intestine using 20150-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20150-1-AP (LGR4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 20150-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20150-1-AP (LGR4 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

20150-1-AP bindet in WB, IHC, ELISA LGR4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LGR4 fusion protein Ag13695
• Vollständiger Name: leucine-rich repeat-containing G protein-coupled receptor 4
• Berechnetes Molekulargewicht: 104 kDa
• GenBank-Zugangsnummer: BC033039
• Gene symbol: LGR4
• Gene ID (NCBI): 55366
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LGR4, also known as GPR48, is a membrane of the leucine-rich GPCR family. LGR4 is composed of a large NH2-terminal extracellular domain containing 18 LRRs, the seven membrane-spanning segments, and a COOH-terminal intracellular domain. LGR4 is a receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. It has also been shown that LGR4 regulates cyclic AMP/protein kinase A (PKA) signalling. (PMID: 23756652; 17178856; 24212090)

Publikationen

Species	Application	Title
mouse	WB	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu
human	WB	Mol Ther Oncolytics; Silencing LGR6 Attenuates Stemness and Chemoresistance via Inhibiting Wnt/β-Catenin Signaling in Ovarian Cancer.; Authors - Xiaohong Ruan
human	IHC	Cancer Biomark; LGR4 overexpression is associated with clinical parameters and poor prognosis of serous ovarian cancer; Authors - Zhaoyang Zeng
human	IHC	Exp Cell Res; Silencing of RSPO1 mitigates obesity-related renal fibrosis in mice by deactivating Wnt/β-catenin pathway.; Authors - Xuesong Su
mouse	WB	In Vitro Cell Dev Biol Anim; SOX2 suppresses osteoblast differentiation of MC3T3-E1 cells through activating the transcription of LGR4; Authors - Sunyu Chen
pig,mouse	WB	Cell Death Dis; DNA methylation mediated RSPO2 to promote follicular development in mammals.; Authors - Xiaofeng Zhou