LGMN Monoklonaler Antikörper

LGMN Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

1F2B12

Kat-Nr. : 67017-1-Ig

Synonyme

AEP, Asparaginyl endopeptidase, legumain, LGMN, LGMN1, Protease, cysteine 1, PRSC1

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Galerie der Validierungsdaten

• 

  WB analysis using 67017-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67017-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67017-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67017-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67017-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig kidney using 67017-1-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67017-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 67017-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig kidney using 67017-1-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 67017-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 67017-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 67017-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 67017-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human kidney using 67017-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LGMN antibody (67017-1-Ig, Clone: 1F2B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human kidney using 67017-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LGMN antibody (67017-1-Ig, Clone: 1F2B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SK-BR-3 cells, ATDC-5-Zellen, Hausschwein-Nierengewebe, HEK-293-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, JAR-Zellen, Jurkat-Zellen, LNCaP-Zellen, MCF-7-Zellen, NCCIT-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Nierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

67017-1-Ig bindet in WB, IHC, IF, ELISA LGMN und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LGMN fusion protein Ag7218
• Vollständiger Name: legumain
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC003061
• Gene symbol: LGMN
• Gene ID (NCBI): 5641
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).

Publikationen

Species	Application	Title
mouse	WB	J Control Release; A "dual-guide" bioinspired drug delivery strategy of a macrophage-based carrier against postoperative triple-negative breast cancer recurrence.; Authors - Yue Qiu
mouse	WB	Int J Pharm; Macrophage-mediated multi-mode drug release system for photothermal combined with anti-inflammatory therapy against postoperative recurrence of triple negative breast cancer.; Authors - Kebai Ren
human	WB	Int J Biol Sci; Legumain Promotes Gastric Cancer Progression Through Tumor-associated Macrophages In vitro and In vivo.; Authors - Hongbin Wang
human	WB,IHC	Exp Eye Res; Legumain affects the PI3K/AKT tumor progression pathway in retinoblastoma; Authors - Qin Tang ; KD Validated
human	IHC	J Mol Neurosci; The Heterogeneity of Tumour-Associated Macrophages Contributes to the Recurrence and Outcomes of Glioblastoma Patients; Authors - Zixue Xuan