P3H2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

P3H2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 15723-1-AP

Synonyme

leprecan like 1, Leprecan like protein 1, LEPREL1, MLAT4, P3H2, Prolyl 3 hydroxylase 2

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Galerie der Validierungsdaten

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  WB analysis using 15723-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 15723-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15723-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 15723-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15723-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 15723-1-AP

  IP Result of anti-P3H2 (IP:15723-1-AP, 4ug; Detection:15723-1-AP 1:600) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human liver using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15723-1-AP (P3H2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15723-1-AP (P3H2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15723-1-AP (P3H2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15723-1-AP (P3H2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, HEK-293-Zellen, humanes Plazenta-Gewebe, Mausnierengewebe, Maus-Plazenta-Gewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Hautgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

15723-1-AP bindet in WB, IP, IHC, ELISA P3H2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P3H2 fusion protein Ag8444
• Vollständiger Name: leprecan-like 1
• Berechnetes Molekulargewicht: 708aa,81 kDa; 527aa,60 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC005029
• Gene symbol: LEPREL1
• Gene ID (NCBI): 55214
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

P3H2 (prolyl 3-hydroxylase 2, also known as LEPREL1) is a member of the leprecan family of proteins, which also include P3H1, P3H3, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H2 shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer.

Publikationen

Species	Application	Title
mouse	WB	J Biol Chem; Post-translationally abnormal collagens of prolyl 3-hydroxylase-2 null mice offer a pathobiological mechanism for the high myopia linked to human LEPREL1 mutations*; Authors - David M Hudson
human	WB	Sci Rep; Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure; Authors - Takahiro Isono
human	WB	J Biol Chem; Post-translational modification of type IV collagen with 3-hydroxyproline affects its interactions with glycoprotein VI and nidogens 1 and 2.; Authors - Nathan T Montgomery ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

We conducted a screening of basal expression levels in multiple human tumor cell lines, attempting antibody concentrations ranging from 1:500 to 2000, all of which exhibited clear bands, indicating stable working conditions. However, the expression depends on the cancer cell line itself.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Multiple cancer cell lines

P3H2 Antibody Western Blot validation (1:500 dilution) in Multiple cancer cell lines (Cat no:15723-1-AP)