LDHC-Specific Polyklonaler Antikörper

LDHC-Specific Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 19989-1-AP

Synonyme

Cancer/testis antigen 32, CT32, lactate dehydrogenase C, LDH C, LDH testis subunit, LDH X, LDH3, LDHC, LDHC-Specific, LDHX

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Galerie der Validierungsdaten

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  WB analysis of human testis using 19989-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 19989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 19989-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 19989-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19989-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 19989-1-AP

  IP Result of anti-LDHC-Specific (IP:19989-1-AP, 4ug; Detection:19989-1-AP 1:500) with mouse testis tissue lysate 2400ug.

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  IHC staining of mouse testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of mouse testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 19989-1-AP

  Immunofluorescent analysis of Hela cells, using LDHC antibody 19989-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, A549-Zellen, Maushirngewebe, Maushodengewebe, Mauslungengewebe, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Herzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

19989-1-AP bindet in WB, IP, IHC, IF, ELISA LDHC-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: lactate dehydrogenase C
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 30-36 kDa
• GenBank-Zugangsnummer: NM_002301
• Gene symbol: LDHC
• Gene ID (NCBI): 3948
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LDHC, also named as CT32 and LDH-X, belongs to the LDH/MDH superfamily and LDH family. LDHC is a possible role in sperm motility. LDHC catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. LDHC is a testis-specific isoform. Abnormal LDHC expression is associated with several forms of cancer. The antibody has no cross reaction with LDHA and LDHB.

Publikationen

Species	Application	Title
mouse	WB	Front Pharmacol; Combination of the Herbs Radix Rehmanniae and Cornus Officinalis Mitigated Testicular Damage From Diabetes Mellitus by Enhancing Glycolysis via the AGEs/RAGE/HIF-1α Axis.; Authors - Yuping Chen
	IHC	Med Sci Monit; PMID: 31332157; Authors - Li Yang
mouse	WB,IHC	Andrology; Generation of humanized LDHC knock-in mice as a tool to assess human LDHC-targeting contraceptive drugs; Authors - Rie Iida-Norita

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (09-23-2023)

Utilizing the concentration of 1:1000, WB results are clear in kinds lung cancer cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Lung cancer cell lines

LDHC-Specific Antibody Western Blot validation (1:1000 dilution) in Lung cancer cell lines (Cat no:19989-1-AP)