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  • Featured Product
  • KD/KO Validated

LDHB Polyklonaler Antikörper

LDHB Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-14824

Synonyme

lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, TRG 5

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated LDHB Polyclonal anti (CL488-14824)

    IF Staining of HeLa using CL488-14824

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 LDHB antibody (CL488-14824) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated LDHB Polyclonal anti (CL488-14824)

    FC experiment of HeLa using CL488-14824

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human LDHB (CL488-14824) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-14824 bindet in IF, FC (Intra) LDHB und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen LDHB fusion protein Ag6605
Vollständiger Name lactate dehydrogenase B
Berechnetes Molekulargewicht 36 kDa
Beobachtetes Molekulargewicht 35 kDa
GenBank-ZugangsnummerBC002362
Gene symbol LDHB
Gene ID (NCBI) 3945
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of pyruvate and NADH to lactate and NAD+ in the glycolytic pathway (PMID: 26269128). It is glycolytic enzymes and it is positively regulated by mTOR.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 LDHB antibody CL488-14824Protokoll herunterladen
FC protocol for CL Plus 488 LDHB antibody CL488-14824Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen