LDHA Monoklonaler Antikörper

LDHA Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

17

CloneNo.

2E2G6

Kat-Nr. : 66287-1-Ig

Synonyme

lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, PIG19

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Galerie der Validierungsdaten

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  WB analysis using 66287-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66287-1-Ig (LDHA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 66287-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66287-1-Ig (LDHA Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66287-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66287-1-Ig (LDHA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66287-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66287-1-Ig (LDHA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66287-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66287-1-Ig (LDHA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66287-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66287-1-Ig(LDHA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Lebergewebe, A431-Zellen, Hausschwein-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

66287-1-Ig bindet in WB, IP, IHC, IF, ELISA LDHA und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LDHA fusion protein Ag21417
• Vollständiger Name: lactate dehydrogenase A
• Berechnetes Molekulargewicht: 332 aa, 37 kDa
• Beobachtetes Molekulargewicht: 32-37 kDa
• GenBank-Zugangsnummer: BC067223
• Gene symbol: LDHA
• Gene ID (NCBI): 3939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies. Mutations in LDHA have been linked to exertional myoglobinuria. LDHA has some isoforms with MW 26-40 kDa.

Publikationen

Species	Application	Title
mouse	WB	Life Sci; Aerobic exercise reduced the amount of CHRONO bound to BMAL1 and ameliorated glucose metabolic dysfunction in skeletal muscle of high-fat diet-fed mice; Authors - Lei Xu
rat	WB	J Cell Mol Med; PMID: 31945804; Authors - Chenyang Chen
mouse	IHC	Int J Biol Sci; LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis.; Authors - Yan Fang
human	WB	Front Oncol; Lactate Dehydrogenase B Is Required for Pancreatic Cancer Cell Immortalization Through Activation of Telomerase Activity.; Authors - Ruiguan Wang
human,mouse	WB,IF	Front Oncol; Ursolic Acid Inhibits Breast Cancer Metastasis by Suppressing Glycolytic Metabolism via Activating SP1/Caveolin-1 Signaling.; Authors - Shengqi Wang
human	IHC	J Cell Mol Med; FOXM1 regulates glycolysis in nasopharyngeal carcinoma cells through PDK1.; Authors - Qing Yang