LDHA Polyklonaler Antikörper

LDHA Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

34

Rezensionen (1)

Kat-Nr. : 21799-1-AP

Synonyme

lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, PIG19

---

Galerie der Validierungsdaten

• 

  WB analysis using 21799-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21799-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21799-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21799-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 21799-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21799-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 21799-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 21799-1-AP

  IP Result of anti-LDHA (IP:21799-1-AP, 4ug; Detection:21799-1-AP 1:500) with HEK-293 cells lysate 2000ug.

• 

  IHC staining of human skeletal muscle using 21799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21799-1-AP (LDHA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 21799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21799-1-AP (LDHA antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21799-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 21799-1-AP (LDHA antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 21799-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA antibody (21799-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 21799-1-AP

  Immunofluorescent analysis of HepG2 cells, using LDHA antibody 21799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A375-Zellen, HepG2-Zellen, humanes Hodengewebe, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:30000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 33 publications below
• IHC: See 10 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

21799-1-AP bindet in WB, IP, IHC, IF, ELISA LDHA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LDHA fusion protein Ag16703
• Vollständiger Name: lactate dehydrogenase A
• Berechnetes Molekulargewicht: 332 aa, 37 kDa
• Beobachtetes Molekulargewicht: 32-37 kDa
• GenBank-Zugangsnummer: BC067223
• Gene symbol: LDHA
• Gene ID (NCBI): 3939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087).

Publikationen

Species	Application	Title
mouse	WB	Cell; A tissue injury sensing and repair pathway distinct from host pathogen defense; Authors - Siqi Liu
human	WB	Sci Transl Med; Loss of miR-31-5p drives hematopoietic stem cell malignant transformation and restoration eliminates leukemia stem cells in mice.; Authors - Biying Zhu
human	WB,IF,IP	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
		Cancer Res; HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc.; Authors - Yinghui Li
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Manfred (Verified Customer) (05-30-2022)

This antibody worked perfectly at 1:1000 dilution. I preferred to do overnight incubation at 4 degrees.

• Applications: Western Blot
• Cell Tissue Type: PBMCs