LC3 Polyklonaler Antikörper

LC3 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-14600

Synonyme

LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL488-14600

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CoraLite® Plus 488 LC3 antibody (CL488-14600) at dilution of 1:200.

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  IF Staining of HepG2 using CL488-14600

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CoraLite® Plus 488 LC3 antibody (CL488-14600) at dilution of 1:200.

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  FC experiment of HeLa using CL488-14600

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human LC3 (CL488-14600) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HEK-293 using CL488-14600

  1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human LC3 (CL488-14600) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: mit Chloroquin behandelte HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-14600 bindet in IF, FC (Intra) LC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LC3 fusion protein Ag6144
• Vollständiger Name: microtubule-associated protein 1 light chain 3 beta
• Berechnetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC067797
• Gene symbol: MAP1LC3B
• Gene ID (NCBI): 81631
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C