LASP1 Monoklonaler Antikörper

LASP1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1G4B6

Kat-Nr. : 68080-1-Ig

Synonyme

Lasp 1, LASP1, LIM and SH3 domain protein 1, LIM and SH3 protein 1, MLN 50, MLN50

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Galerie der Validierungsdaten

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  WB analysis using 68080-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68080-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood platelets using 68080-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 68080-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 68080-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 68080-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 68080-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 68080-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LASP1 antibody (68080-1-Ig, Clone: 1G4B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HCT 116-Zellen, HeLa-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

68080-1-Ig bindet in WB, IHC, IF, ELISA LASP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LASP1 fusion protein Ag18101
• Vollständiger Name: LIM and SH3 protein 1
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC012460
• Gene symbol: LASP1
• Gene ID (NCBI): 3927
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LASP1(LIM and SH3 protein 1), also known as MLN50, is a 261 amino acid protein that localizes to both the cytoplasm and the cytoskeleton(PMID: 7589475). LASP1 consists of an N-terminal LIM-domain with two zinc finger motifs, followed by two central actin-binding nebulin repeats, flanked by a linker region and a C-terminal SH3 domain (PMID: 17177073, 9848085). LASP-1 interacts with F-Actin and plays an important role in the regulation of Actin-associated cytoskeletal organization. Agonist-dependent changes in LASP1 phosphorylation may regulate Actin-related ion transport activities in epithelial cells (PMID: 15465019,12571245). Overexpression of LASP-1 is associated with breast cancer, and plays a role in tumor transformation and metastasis (PMID: 17956604).

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; TRIM15 forms a regulatory loop with the AKT/FOXO1 axis and LASP1 to modulate the sensitivity of HCC cells to TKIs; Authors - Chong Yang ; KD Validated