LARP7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LARP7 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Rezensionen (1)

Kat-Nr. : 17067-1-AP

Synonyme

HDCMA18P, hLARP7, La related protein 7, La-related protein 7, PIP7S

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  WB analysis using 17067-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17067-1-AP (LARP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17067-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17067-1-AP (LARP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17067-1-AP (LARP7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17067-1-AP (LARP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17067-1-AP (LARP7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17067-1-AP (LARP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17067-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LARP7 antibody (17067-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Hirngewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lebergewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Magenkrebsgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

17067-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA LARP7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LARP7 fusion protein Ag10891
• Vollständiger Name: La ribonucleoprotein domain family, member 7
• Berechnetes Molekulargewicht: 582 aa, 67 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC006981
• Gene symbol: LARP7
• Gene ID (NCBI): 51574
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LARP7 belongs to the LARP RNA-binding protein family, which includes the lupus LA antigen gene, and modulates the metabolism and function of a variety of RNA species [PMID:20138158]. LARP7 binds to the 7SK RNP complex, and sequesters the positive transcription elongation factor b (P-TEFb) in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. binds to and stabilizes 7sk snRNA[PMID:19416841].

Publikationen

Species	Application	Title
mouse	WB	Genes Dev; Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells.; Authors - Daiji Okamura
mouse,human	IP,IF	Nat Commun; Interaction of 7SK with the Smn complex modulates snRNP production.; Authors - Changhe Ji ; KD Validated
human	WB	Nucleic Acids Res; 7SK small nuclear RNA directly affects HMGA1 function in transcription regulation.; Authors - Eilebrecht Sebastian S
human	WB	Nucleic Acids Res; HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters.; Authors - Sebastian Eilebrecht
human	WB	EMBO Rep; Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs.; Authors - Ahmed S Warda
human	WB,IP,IF	MBio; Interaction of the Ankyrin H Core Effector of Legionella with the Host LARP7 Component of the 7SK snRNP Complex.; Authors - Juanita Von Dwingelo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Monika (Verified Customer) (10-09-2023)

Great antibody, successfully used in western blots with 1:1000 to 1:2000 dilutions. Verified using a Calu-3 and HEK293T LARP7 knock-out cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: lung carcinoma, Calu-3 cells

LARP7 Antibody Western Blot validation (1:1000 dilution) in lung carcinoma, Calu-3 cells (Cat no:17067-1-AP)