LARG Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LARG Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 22441-1-AP

Synonyme

ARHGEF12, KIAA0382, LARG, Leukemia associated RhoGEF, PRO2792

---

Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 22441-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22441-1-AP (LARG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22441-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22441-1-AP (LARG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 22441-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22441-1-AP (LARG Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 22441-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22441-1-AP (LARG Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 22441-1-AP

  Immunofluorescent analysis of HepG2 cells, using ARHGEF12 antibody 22441-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 22441-1-AP

  Immunofluorescent analysis of HeLa cells using 22441-1-AP (LARG antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

22441-1-AP bindet in WB, IHC, IF, ELISA LARG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LARG fusion protein Ag18211
• Vollständiger Name: Rho guanine nucleotide exchange factor (GEF) 12
• Berechnetes Molekulargewicht: 1544 aa, 173 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: BC063117
• Gene symbol: ARHGEF12
• Gene ID (NCBI): 23365
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LARG, also known as ARHGEF12, is one of three regulator of G protein signalling (RGS) domain-containing RhoGEFs. LARG activates the ROCK pathway downstream of Gα12/13 signaling, leading to cytoskeletal reorganisation. LARG performs this activity either as a homodimer or as a heterodimer with Arhgef11. LARG has been implicated in many diseases, including cancer, heart disease, and in HIV viral replication. LARG is phosphorylated and dephosphorylated during mitosis and that Cdk1 is the mitotic kinase responsible for LARG phosphorylation. We got 220 kDa in western blotting, maybe due to its phosphorylation.

Publikationen

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; RSK2 drives cell motility by serine phosphorylation of LARG and activation of Rho GTPases.; Authors - Geng-Xian Shi
human	WB	Br J Pharmacol; Reregulation of hepatic stellate cell contraction and cirrhotic portal hypertension by Wnt/β-catenin signaling via interaction with Gli1.; Authors - Feng Zhang
human	WB,IHC	Am J Pathol; The Guanine Nucleotide Exchange Factor, Leukemia-Associated Rho Guanine Nucleotide Exchange Factor, and RhoC Play a Role in Glioblastoma Cell Invasion and Resistance.; Authors - Zonghui Ding ; KD Validated
mouse	WB	Int J Mol Sci; Inhibition of High-Temperature Requirement Protein A2 Protease Activity Represses Myogenic Differentiation via UPRmt; Authors - Hongyu Sun