LAPTM4B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LAPTM4B Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 18895-1-AP

Synonyme

LAPTM4B, LAPTM4beta, LC27

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse lymph using 18895-1-AP

  mouse lymph tissue were subjected to SDS PAGE followed by western blot with 18895-1-AP (LAPTM4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lymph using 18895-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 18895-1-AP (LAPTM4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 18895-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 18895-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18895-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18895-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Lymphgewebe, Rattenlymphgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Produktinformation

18895-1-AP bindet in WB, IHC, IF, ELISA LAPTM4B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LAPTM4B fusion protein Ag13521
• Vollständiger Name: lysosomal protein transmembrane 4 beta
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC014129
• Gene symbol: LAPTM4B
• Gene ID (NCBI): 55353
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAPTM4B, a novel oncoprotein, is associated with the prognosis of cancer, and may play important roles in the initiation, promotion and metastasis of tumors. It was initially identified as a hepatocellular carcinoma-associated gene, and was recently shown to be up-regulated in various human cancers. Some studies indicated that LAPTM4B may be an useful marker of prognosis in ovarian carcinoma and endometrial cancer. LAPTM4B belongs to the LAPTM4/LAPTM5 transporter family. It has 3 isoforms with molecular masses 41 kDa, 35 kDa and 24 kDa. This antibody can recognize all the 3 isoforms of LAPTM4B.

Publikationen

Species	Application	Title
human	WB, IF	Cell; A kinase-independent role for EGF receptor in autophagy initiation.; Authors - Xiaojun Tan ; KD Validated
	WB	Nat Commun; LAPTM4b recruits the LAT1-4F2hc Leu transporter to lysosomes and promotes mTORC1 activation.; Authors - Ruth Milkereit
human	IF	Proc Natl Acad Sci U S A; PtdIns(4,5)P2 signaling regulates ATG14 and autophagy.; Authors - Xiaojun Tan
	IHC	Front Cell Dev Biol; Integrative Genome-Wide DNA Methylome and Transcriptome Analysis of Ovaries from Hu Sheep with High and Low Prolific.; Authors - Xiaolei Yao
human	WB,IHC,IF	Biochem Biophys Res Commun; Helicobacter pylori infection promotes epithelial-to-mesenchymal transition of gastric cells by upregulating LAPTM4B.; Authors - Shengfei Zhou ; KD Validated
human	IF	Cancer Manag Res; LAPTM4B facilitates tumor growth and induces autophagy in hepatocellular carcinoma.; Authors - Fei Wang