LAP3 Polyklonaler Antikörper

LAP3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 14612-1-AP

Synonyme

Cytosol aminopeptidase, LAP, LAP 3, LAP3, LAPEP, leucine aminopeptidase 3, Leucyl aminopeptidase, PEPS, Peptidase S, Proline aminopeptidase, Prolyl aminopeptidase

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14612-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14612-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 14612-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 14612-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14612-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 14612-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14612-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 14612-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14612-1-AP (LAP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14612-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14612-1-AP (LAP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Mausherzgewebe, Mauslungengewebe, Mausmilzgewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

14612-1-AP bindet in WB, IHC, ELISA LAP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LAP3 fusion protein Ag6205
• Vollständiger Name: leucine aminopeptidase 3
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC065564
• Gene symbol: LAP3
• Gene ID (NCBI): 51056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAP3(Leucine aminopeptidase 3) is also named as LAPEP, PEPS(peptidase S) and belongs to the peptidase M17 family. It can catalyzes the removal of unsubstituted N-terminal amino-acids from various peptides and be presumably involved in the processing end regular turnover of intracellular proteins. PEPS is found in a wide variety of tissues and cultured cells but not in red cells and skin. The enzyme can utilize several di-, tri-, and tetrapeptides as substrates and is the slowest migrating of the peptidases after electrophoresis.(PMID:689684).

Publikationen

Species	Application	Title
mouse	WB	Int J Biochem Cell Biol; The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis.; Authors - Zhu Zijue Z
rat	WB	Reprod Toxicol; 2,2',4,4'-Tetrabromodiphenyl ether disrupts spermatogenesis, impairs mitochondrial function and induces apoptosis of early leptotene spermatocytes in rats.; Authors - Shaoping Huang
human	WB	Cancer Genomics Proteomics; Proteomic Analysis of Hepatocellular Carcinoma Tissues With Encapsulation Shows Up-regulation of Leucine Aminopeptidase 3 and Phosphoenolpyruvate Carboxykinase 2.; Authors - Keisuke Kuhara