LACTB Polyklonaler Antikörper

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• KD/KO Validated

LACTB Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 18195-1-AP

Synonyme

G24, lactamase, beta, LACTB, MRPL56

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 18195-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18195-1-AP (LACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18195-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18195-1-AP (LACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 18195-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18195-1-AP (LACTB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18195-1-AP (LACTB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18195-1-AP (LACTB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18195-1-AP (LACTB antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 18195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18195-1-AP (LACTB antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 18195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 18195-1-AP (LACTB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, humanes Herzgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 4 publications below

Produktinformation

18195-1-AP bindet in WB, IHC, IF, ELISA LACTB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LACTB fusion protein Ag12233
• Vollständiger Name: lactamase, beta
• Berechnetes Molekulargewicht: 547 aa, 61 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC067288
• Gene symbol: LACTB
• Gene ID (NCBI): 114294
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LACTB is also named as MRPL56,which is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome.LACTB is a mammalian active-site serine protein that has evolved from a bacterial penicillin-binding protein.It promotes intramitochondrial membrane organization and micro-compartmentalization through polymerization(PMID:19858488). Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen(PMID:11707067). The full-length of LACTB is about 61kDa, and a 115aa transit peptide can be removed in the mature form. This antibody is specific to LACTB.

Publikationen

Species	Application	Title
human	WB,IHC,IF	Nature; LACTB is a tumour suppressor that modulates lipid metabolism and cell state.; Authors - Zuzana Keckesova
human	WB,IHC,IF	Oncogene; LACTB, a novel epigenetic silenced tumor suppressor, inhibits colorectal cancer progression by attenuating MDM2-mediated p53 ubiquitination and degradation.; Authors - Kaixuan Zeng ; KO Validated
Human	IHC	PLoS One; LACTB mRNA expression is increased in pancreatic adenocarcinoma and high expression indicates a poor prognosis.; Authors - Jian Xie
human	IHC	Cancer Biother Radiopharm; Clinical Significance of β-Lactamase Expression in Colorectal Cancer.; Authors - Shaofei Zhou
human	WB	Exp Ther Med; LACTB suppresses carcinogenesis in lung cancer and regulates the EMT pathway.; Authors - Yihui Xu ; KD Validated
human	WB,IF	Apoptosis; LACTB induces cancer cell death through the activation of the intrinsic caspase-independent pathway in breast cancer; Authors - Juan M Gonzalez-Morena