KTELC1 Polyklonaler Antikörper

KTELC1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24409-1-AP

Synonyme

C3orf9, CAP10 like 46 kDa protein, CLP46, hCLP46, KDELCL1, KTELC1, MDS010, MDSRP, UNQ490/PRO1006

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 24409-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24409-1-AP (KTELC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24409-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24409-1-AP (KTELC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24409-1-AP

  Hela cells were subjected to SDS PAGE followed by western blot with 24409-1-AP (KTELC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 24409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24409-1-AP (KTELC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 24409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24409-1-AP (KTELC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 24409-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using KTELC1 antibody (24409-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of C2C12 using 24409-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using KTELC1 antibody (24409-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24409-1-AP bindet in WB, IHC, IF, ELISA KTELC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KTELC1 fusion protein Ag19942
• Vollständiger Name: KTEL (Lys-Tyr-Glu-Leu) containing 1
• Berechnetes Molekulargewicht: 392 aa, 46 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC048810
• Gene symbol: KTELC1
• Gene ID (NCBI): 56983
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KTELC1 also termed as CLP46 is a glucosyltransferase, which implicates in Protein modification and glycosylation. KTELC1 encodes a 392 amino acids protein and its molecular weight is 46kda, but is exists glycosylation modification. KTELC1 is localized in endoplasmic reticulum lumen and abundantly expressed in liver, brain, heart, skeletal muscle, spleen, kidney, placenta, lung and peripheral blood leukocyte. KTELC1 may regulation in cell proliferation and Notch signal pathway.1