KRT80 Polyklonaler Antikörper

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• KD/KO Validated

KRT80 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 16835-1-AP

Synonyme

CK 80, Cytokeratin 80, K80, KB20, keratin 80, KRT80, Type II keratin Kb20

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 16835-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16835-1-AP (KRT80 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 16835-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16835-1-AP (KRT80 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16835-1-AP (KRT80 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16835-1-AP (KRT80 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16835-1-AP (KRT80 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16835-1-AP (KRT80 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3600
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 5 publications below

Produktinformation

16835-1-AP bindet in WB, IHC, IF, ELISA KRT80 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KRT80 fusion protein Ag10498
• Vollständiger Name: keratin 80
• Berechnetes Molekulargewicht: 422 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa, 50 kDa
• GenBank-Zugangsnummer: BC065180
• Gene symbol: KRT80
• Gene ID (NCBI): 144501
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KRT80 is a human IF type II epithelial keratin gene involved in the formation of IF heterodimers in various epithelial cells. KRT80 is overexpressed in many neoplasms and plays an essential role in promoting cell proliferation, migration and invasiveness, and is associated with poor prognosis in cancer patients. KRT80 has three isoforms with predicted MW of 50 kDa, 47 kDa, and 54 kDa.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.; Authors - Daniel Arango
human	IHC	Cell Discov; Single-cell atlas of keratoconus corneas revealed aberrant transcriptional signatures and implicated mechanical stretch as a trigger for keratoconus pathogenesis.; Authors - Shengqian Dou
human	IF	Int J Mol Sci; Keratin Profiling by Single-Cell RNA-Sequencing Identifies Human Prostate Stem Cell Lineage Hierarchy and Cancer Stem-Like Cells.; Authors - Wen-Yang Hu
human	WB,IF	Cell Death Dis; Keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with PRKDC via activating the AKT pathway.; Authors - Changcan Li
human	WB	Cell Cycle; MiR-4268 suppresses gastric cancer genesis through inhibiting keratin 80.; Authors - Fan Zhang ; KD Validated
human	WB,IHC	J Cancer; Keratin 80 regulated by miR-206/ETS1 promotes tumor progression via the MEK/ERK pathway in ovarian cancer.; Authors - Ouxuan Liu