KRT23 Polyklonaler Antikörper

KRT23 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 24049-1-AP

Synonyme

CK 23, CK23, Cytokeratin 23, HAIK1, K23, Keratin 23, KRT23

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Galerie der Validierungsdaten

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  WB analysis of BxPC-3 using 24049-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 24049-1-AP (KRT23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 24049-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24049-1-AP (KRT23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24049-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24049-1-AP (KRT23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Hautkrebsgewebe, humanes Leberkarzinomgewebe, humanes Plazenta-Gewebe, Maus-Kolongewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

24049-1-AP bindet in WB, IHC, IF, ELISA KRT23 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KRT23 fusion protein Ag21129
• Vollständiger Name: keratin 23 (histone deacetylase inducible)
• Berechnetes Molekulargewicht: 422 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC028356
• Gene symbol: KRT23
• Gene ID (NCBI): 25984
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT23 belongs to type I keratins.

Publikationen

Species	Application	Title
human	IF	Cell Rep; Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model.; Authors - Masatoshi Ohgushi