Cytokeratin 19 Monoklonaler Antikörper

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• KD/KO Validated

Cytokeratin 19 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

3G1E4

Kat-Nr. : 60187-1-Ig

Synonyme

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

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Galerie der Validierungsdaten

• 

  WB analysis of MCF-7 using 60187-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60187-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of HeLa using 60187-1-Ig

  WB result of KRT19 antibody (60187-1-IG, 1:10,000) with si-Control and si-KRT19 transfected HeLa cells.

• 

  WB analysis of HaCaT using 60187-1-Ig

  HaCaT cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 60187-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 60187-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 60187-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 60187-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 19 (60187-1-Ig, Clone:3G1E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HaCaT-Zellen, Hausschwein-Kolongewebe, HeLa-Zellen, Maus-Kolongewebe, PC-3-Zellen, Ratten-Kolongewebe, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Lungenkarzinomgewebe, humanes Magenkrebsgewebe, humanes Mammakarzinomgewebe, humanes Ösophaguskarzinomgewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:1000-1:51200
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

60187-1-Ig bindet in WB, IHC, IF, FC, ELISA Cytokeratin 19 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 19 fusion protein Ag7407
• Vollständiger Name: keratin 19
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC002539
• Gene symbol: KRT19
• Gene ID (NCBI): 3880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy; Authors - Xiaoyu Liu
human	WB	Stem Cells; Annexin A3 as a Potential Target for Immunotherapy of Liver Cancer Stem-Like Cells.; Authors - Qiu-Zhong Pan
human	IF	Breast Cancer Res; Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors; Authors - Wei Yang
mouse	IF	Biomed Pharmacother; Serotonin-RhoA/ROCK axis promotes acinar-to-ductal metaplasia in caerulein-induced chronic pancreatitis.; Authors - Xufeng Tao
mouse	IF	Biochim Biophys Acta Mol Basis Dis; TGF-β/YB-1/Atg7 axis promotes the proliferation of hepatic progenitor cells and liver fibrogenesis; Authors - Yuecheng Guo
human	IHC	Reprod Sci; Identification of Key Differentially Methylated/Expressed Genes and Pathways for Ovarian Endometriosis by Bioinformatics Analysis; Authors - Haibo Zhang