Cytokeratin 18 Polyklonaler Antikörper

Cytokeratin 18 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 18708-1-AP

Synonyme

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

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Galerie der Validierungsdaten

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  WB analysis of A431 using 18708-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 18708-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18708-1-AP (Cytokeratin 18 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18708-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 18708-1-AP

  IP Result of anti-Cytokeratin 18 (IP:18708-1-AP, 4ug; Detection:18708-1-AP 1:500) with A431 cells lysate 2000ug.

• 

  IHC staining of human renal cell carcinoma using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human kidney using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human endometrial cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human nephroblastoma using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human nephroblastoma using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human nephroblastoma using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human nephroblastoma using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 18708-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, COLO 320-Zellen, HepG2-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe, humanes Endometriumkarzinomgewebe, humanes Hautkrebsgewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe, humanes Mammakarzinomgewebe, humanes Nephroblastomgewebe, humanes Nierengewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

18708-1-AP bindet in WB, IP, IHC, IF, ELISA Cytokeratin 18 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Hausschwein, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: keratin 18
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000180
• Gene symbol: KRT18
• Gene ID (NCBI): 3875
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. This antibody is specifically agianst KRT18.

Publikationen

Species	Application	Title
mouse	IHC	Oncoscience; The helix-loop-helix transcriptional regulator Id4 is required for terminal differentiation of luminal epithelial cells in the prostate.; Authors - Dhanushka Hewa Bostanthirige
bovine	IF	J Agric Food Chem; CIDEA Regulates De Novo Fatty Acid Synthesis in Bovine Mammary Epithelial Cells by Targeting the AMPK/PPARγ Axis and Regulating SREBP1; Authors - Ji Cheng
pig	IHC	Cells; Hypothalamic-Pituitary-Adrenal Hormones Impair Pig Fertilization and Preimplantation Embryo Development via Inducing Oviductal Epithelial Apoptosis: An In Vitro Study; Authors - Jin-Song An
bovine	IF	J Agric Food Chem; Mitochondrial Calcium Uniporter Regulator 1 (MCUR1) Relieves Mitochondrial Damage Induced by Lipopolysaccharide by Mediating Mitochondrial Ca2+ Homeostasis in Bovine Mammary Epithelial Cells; Authors - Xudong Sun