Cytokeratin 14 Monoklonaler Antikörper

Cytokeratin 14 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

CloneNo.

2G1E2

Kat-Nr. : 60320-1-Ig

Synonyme

Cell and organelle markers, CK 14, CK14, Cytokeratin 14, Cytoskeleton Marker, EBS3, EBS4, K14, keratin 14, KRT14, NFJ

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Galerie der Validierungsdaten

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  WB analysis of A431 using 60320-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60320-1-Ig (Cytokeratin 14 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 60320-1-Ig

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 60320-1-Ig (Cytokeratin 14 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:1600 (under 1010x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60320-1-Ig (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 60320-1-Ig (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 60320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 60320-1-Ig (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A431 using 60320-1-Ig

  Immunofluorescent analysis of A431 cells using 60320-1-Ig (Cytokeratin 14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

• 

  IF Staining of mouse skin using 60320-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using Cytokeratin 14 antibody (60320-1-Ig, Clone: 2G1E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse skin using 60320-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using Cytokeratin 14 antibody (60320-1-Ig, Clone: 2G1E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Maushautgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Hautgewebe, humanes Hautkrebsgewebe, humanes Mammahyperplasie-Gewebe, humanes Zervixkarzinomgewebe, Rattenhautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen, Maushautgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:400-1:800
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 12 publications below

Produktinformation

60320-1-Ig bindet in WB, IHC, IF, ELISA Cytokeratin 14 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 14 fusion protein Ag17559
• Vollständiger Name: keratin 14
• Berechnetes Molekulargewicht: 472 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC002690
• Gene symbol: KRT14
• Gene ID (NCBI): 3861
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

Publikationen

Species	Application	Title
mouse	IF	Small; Eco-Friendly and Scalable Synthesis of Fullerenols with High Free Radical Scavenging Ability for Skin Radioprotection.; Authors - Maoru Zhao
human	WB	Breast Cancer Res; Anillin regulates breast cancer cell migration, growth, and metastasis by non-canonical mechanisms involving control of cell stemness and differentiation.; Authors - Dongdong Wang
mouse	WB,IHC	Environ Sci Technol; Graphene Quantum Dots Disrupt Embryonic Stem Cell Differentiation by Interfering with the Methylation Level of Sox2.; Authors - Tingting Ku
mouse	IF	Stem Cell Reports; SOX2 Is a Marker for Stem-like Tumor Cells in Bladder Cancer.; Authors - Fengyu Zhu
human	IF	J Nanobiotechnology; Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration.; Authors - Yunyao Bo
mouse	IF	Stem Cell Res Ther; Bone marrow mesenchymal stem cells facilitate diabetic wound healing through the restoration of epidermal cell autophagy via the HIF-1α/TGF-β1/SMAD pathway; Authors - Yan Shi