Cytokeratin 10-specific Polyklonaler Antikörper

Cytokeratin 10-specific Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16855-1-AP

Synonyme

Cell and organelle markers, CK 10, CK10, Cytokeratin 10, Cytokeratin 10-specific, Cytoskeleton Marker, K10, keratin 10, KPP, KRT10

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Galerie der Validierungsdaten

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  WB analysis using 16855-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A431 using 16855-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.5 ug Anti-Human Cytokeratin 10-specific (16855-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe, Rattenhautgewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Mammakarzinomgewebe, Maushautgewebe, Rattenhautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16855-1-AP bindet in WB, IHC, FC, ELISA Cytokeratin 10-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: keratin 10
• Berechnetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: NM_000421
• Gene symbol: KRT10
• Gene ID (NCBI): 3858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia. This antibody is specifically against KRT10.