pan Ras Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

pan Ras Monoklonal Antikörper für FC (Intra), IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Huhn, human, Kaninchen, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

13

CloneNo.

4H4G7

Kat-Nr. : 60309-1-Ig

Synonyme

C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2B speicifc, Krasa, Krasb, NS3, pan Ras, RASK2

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Galerie der Validierungsdaten

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  WB analysis using 60309-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60309-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60309-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 60309-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 60309-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 60309-1-Ig

  IP result of anti-pan Ras (IP:60309-1-Ig, 5ug; Detection:60309-1-Ig 1:2000) with mouse brain tissue lysate 4000 ug.

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  IHC staining of human breast cancer using 60309-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60309-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60309-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 60309-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 60309-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60309-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 60309-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human pan Ras (60309-1-Ig, Clone:4H4G7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, Hausschwein-Hirngewebe, HEK-293-Zellen, Hühnerhirngewebe, humanes Hirngewebe, Kaninchenhirngewebe, Maushirngewebe, Neuro-2a-Zellen, Rattenhirngewebe, ROS1728-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

60309-1-Ig bindet in WB, IP, IHC, IF, FC (Intra), ELISA pan Ras und zeigt Reaktivität mit Hausschwein, Huhn, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: pan Ras fusion protein Ag2700
• Vollständiger Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Berechnetes Molekulargewicht: 188 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC013572
• Gene symbol: KRAS
• Gene ID (NCBI): 3845
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8+ memory T cells.; Authors - Huafeng Zhang
human	WB	Cancer Res; KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis.; Authors - Peng Yuan ; KD Validated
human	IHC	Exp Mol Med; Destabilization of β-catenin and RAS by targeting the Wnt/β-catenin pathway as a potential treatment for triple-negative breast cancer.; Authors - Won-Ji Ryu
human	WB	J Cell Mol Med; DEPDC1 up-regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells.; Authors - Wei Wang
human	WB	Mol Pharm; Hyaluronic Acid-Modified Nanoparticles Self-Assembled from Linoleic Acid-Conjugated Chitosan for the Codelivery of miR34a and Doxorubicin in Resistant Breast Cancer; Authors - Xiaoxia Yang
human	WB	Oncol Rep; EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non‑small cell lung cancer cells.; Authors - Chuan Lin