KMT2D Polyklonaler Antikörper

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• KD/KO Validated

KMT2D Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 27266-1-AP

Synonyme

AAD10, ALL1 related protein, ALR, CAGL114, KMT2D, Lysine N methyltransferase 2B, MLL2, MLL4, TNRC21

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Galerie der Validierungsdaten

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  WB analysis using 27266-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27266-1-AP (KMT2D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• ChIP: See 2 publications below

Produktinformation

27266-1-AP bindet in WB, IHC, IF, ChIP, ELISA KMT2D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KMT2D fusion protein Ag26195
• Vollständiger Name: myeloid/lymphoid or mixed-lineage leukemia 2
• Berechnetes Molekulargewicht: 593 kDa
• Beobachtetes Molekulargewicht: 593 kDa
• GenBank-Zugangsnummer: NM_003482
• Gene symbol: MLL2
• Gene ID (NCBI): 8085
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KMT2D (a COMPASS/ Set1 family member; also known as MLL4, ALR, and MLL2) is the biggest H3K4 methyltransferase among the most frequently mutated genes in many different types of cancer (PMID: 34194626). The enzymatic function of KMT2D depends on a cluster of C-terminal conserved domains, including a PHD domain, two FY-rich motifs (FYRC and FYRN) and a catalytic SET domain. KMT2D has two frequently occurring genomic alterations: truncation and missense mutations. Loss of KMT2D is a bona fide tumor suppressor gene in FL and DLBCL. Kmt2d perturbed the expression of genes that sustain proliferation and survival, and the KMT2D protein directly binds and associates with an active chromatin conformation in negative modulators of the BCR and lymphocyte migration pathways, which in turn could affect B cell responses to antigen(PMID: 26366712).

Publikationen

Species	Application	Title
human	WB	Cancer Immunol Res; Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing-Assisted Multiomics Analysis.; Authors - Xuanwen Bao
human	IHC	Oncogene; Histone 3 lysine-27 demethylase KDM6A coordinates with KMT2B to play an oncogenic role in NSCLC by regulating H3K4me3.; Authors - Xuejiao Leng
human	IHC,IF	Cell Biosci; Histone methyltransferase KMT2D cooperates with MEF2A to promote the stem-like properties of oral squamous cell carcinoma.; Authors - Xinmiao Wang
human	IHC	Thorac Cancer; RNF213 gene mutation in circulating tumor DNA detected by targeted next-generation sequencing in the assisted discrimination of early-stage lung cancer from pulmonary nodules.; Authors - Ning Jiang
	ChIP	Cell Death Discov; Pseudogene ACTBP2 increases blood-brain barrier permeability by promoting KHDRBS2 transcription through recruitment of KMT2D/WDR5 in Aβ1-42 microenvironment.; Authors - Qianshuo Liu
human	WB,IHC,IF	World J Gastrointest Oncol; KMT2D deficiency enhances the anti-cancer activity of L48H37 in pancreatic ductal adenocarcinoma.; Authors - Si-Si Li ; KD Validated