KLHL3 Polyklonaler Antikörper

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• KD/KO Validated

KLHL3 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 16951-1-AP

Synonyme

kelch like 3 (Drosophila), Kelch like protein 3, KIAA1129, KLHL3

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 16951-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16951-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16951-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16951-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16951-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16951-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 16951-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 16951-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 16951-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16951-1-AP

  IP Result of anti-KLHL3 (IP:16951-1-AP, 4ug; Detection:16951-1-AP 1:700) with HepG2 cells lysate 2800ug.

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  IHC staining of human liver using 16951-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16951-1-AP (KLHL3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16951-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16951-1-AP (KLHL3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human tonsillitis using 16951-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 16951-1-AP (KLHL3 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 16951-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 16951-1-AP (KLHL3 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HL-60-Zellen, humanes Lebergewebe, Jurkat-Zellen, Mauslebergewebe, Maus-Pankreasgewebe, Maus-Thymusgewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

16951-1-AP bindet in WB, IP, IHC, IF, ELISA KLHL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KLHL3 fusion protein Ag10580
• Vollständiger Name: kelch-like 3 (Drosophila)
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC034035
• Gene symbol: KLHL3
• Gene ID (NCBI): 26249
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IHC	Nat Genet; KLHL3 mutations cause familial hyperkalemic hypertension by impairing ion transport in the distal nephron.; Authors - Louis-Dit-Picard Hélène H
human	WB,IHC	Cell Death Differ; A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA.; Authors - Xiaoyu Qi
mouse	WB,IF	J Am Soc Nephrol; Combined Kelch-Like 3 and Cullin 3 Degradation is a Central Mechanism in Familial Hyperkalemic Hypertension in Mice.; Authors - Yujiro Maeoka
mouse	WB	J Am Soc Nephrol; Kelch-Like Protein 2 Mediates Angiotensin II-With No Lysine 3 Signaling in the Regulation of Vascular Tonus.; Authors - Moko Zeniya
mouse	WB	J Am Soc Nephrol; Renal COP9 Signalosome Deficiency Alters CUL3-KLHL3-WNK Signaling Pathway.; Authors - Ryan J Cornelius
mouse	IF	Hum Mol Genet; Impaired degradation of WNK1 and WNK4 kinases causes PHAII in mutant KLHL3 knock-in mice.; Authors - Koichiro Susa