KISS1R-Specific Polyklonaler Antikörper

KISS1R-Specific Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15505-1-AP

Synonyme

AXOR12, G protein coupled receptor 54, GPR54, HOT7T175, Hypogonadotropin 1, KiSS 1 receptor, KiSS 1R, KISS1 receptor, KISS1R, KISS1R-Specific, Kisspeptins receptor, Metastin receptor

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Galerie der Validierungsdaten

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  WB analysis of mouse cerebellum using 15505-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 15505-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15505-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 15505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 15505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 15505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15505-1-AP

  Immunofluorescent analysis of MCF-7 cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 15505-1-AP

  Immunofluorescent analysis of Hela cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 15505-1-AP

  Immunofluorescent analysis of HepG2 cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of SH-SY5Y using 15505-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug KISS1R-Specific antibody (15505-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, MCF-7-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

15505-1-AP bindet in WB, IHC, IF, FC, ELISA KISS1R-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: KISS1 receptor
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 43-45 kDa
• GenBank-Zugangsnummer: NM_032551
• Gene symbol: KISS1R
• Gene ID (NCBI): 84634
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KISS1R is the receptor for metastin (kisspeptin-54, 13, 14, 10), C- terminally amidated peptides of KISS-1. KISS-1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some other carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.

Publikationen

Species	Application	Title
		J Cell Physiol; Kp-10 promotes bovine mammary epithelial cell proliferation by activating GPR54 and its downstream signaling pathways.; Authors - Yanwei Li
bovine	WB,IF	J Agric Food Chem; Kisspeptin-10 Maintains the Activation of the mTOR Signaling Pathway by Inhibiting SIRT6 to Promote the Synthesis of Milk in Bovine Mammary Epithelial Cells.; Authors - Yu Cao
bovine	WB	Int J Mol Sci; Kisspeptin-10 Induces β-Casein Synthesis via GPR54 and Its Downstream Signaling Pathways in Bovine Mammary Epithelial Cells.; Authors - Jianhua Sun
human	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen