KIF5C Polyklonaler Antikörper

KIF5C Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 25897-1-AP

Synonyme

KIAA0531, KIF5C, kinesin family member 5C, Kinesin heavy chain isoform 5C, KINN, NKHC, NKHC 2, NKHC2

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Galerie der Validierungsdaten

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  WB analysis using 25897-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25897-1-AP (KIF5C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25897-1-AP

  IP result of anti-KIF5C(IP:25897-1-AP, 4ug; Detection:25897-1-AP 1:800) with mouse brain tissue lysate 2080 ug.

• 

  IHC staining of mouse brain using 25897-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25897-1-AP (KIF5C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25897-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25897-1-AP (KIF5C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 25897-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 25897-1-AP (KIF5C antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

25897-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA KIF5C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF5C fusion protein Ag23163
• Vollständiger Name: kinesin family member 5C
• Berechnetes Molekulargewicht: 725 aa, 83 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 110 kDa
• GenBank-Zugangsnummer: BC110287
• Gene symbol: KIF5C
• Gene ID (NCBI): 3800
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF5C (Kinesin heavy chain isoform 5C variant) which belongs to the kinesin-like protein family is neuron specific. The kinesin-1 subfamilies (kif5a, kif5b, kif5c) are responsible for the movement of mitochondria in neurons. Kinesins have N-terminal motor domains and C-terminal cargo-binding tail domains separated by hinge regions. The hinge and C-terminal tail regions of Kif5a, Kif5b, and Kif5c bound a large detergent-resistant RNase-sensitive granule. The granule localized to dendrites and underwent bidirectional movement. Distally directed movement of the granule was enhanced by Kif5 overexpression and reduced by Kif5 functional blockage. kinesins may transport RNA in dendrites via this large granule. Two major isoforms of kif5c, with molecular predicted masses of 80 and 110 kDa, are generated by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	Exp Neurol; Axonal injury mediated by neuronal p75NTR/TRAF6/JNK pathway contributes to cognitive impairment after repetitive mTBI; Authors - Gang Liu
human	WB	Hum Mol Genet; Mitochondrial transport mediates survival of retinal ganglion cells in affected LHON patients.; Authors - Tien-Chun Yang
mouse	WB	Alzheimers Res Ther; Normal levels of KIF5 but reduced KLC1 levels in both Alzheimer disease and Alzheimer disease in Down syndrome: evidence suggesting defects in anterograde transport.; Authors - Xu-Qiao Chen
human	WB	bioRxiv; MFN2-dependent recruitment of ATAT1 coordinates mitochondria motility with alpha-tubulin acetylation and is disrupted in CMT2A; Authors - Atul Kumar
human	WB,IHC	Int Endod J; Overexpression of MicroRNA-155 Aggravates Pulpitis by Targeting Kinesin Superfamily Proteins-5C Based on Illumina High-Throughput Sequencing; Authors - Ye Jiang ; KD Validated
mouse	WB	Exp Mol Med; Activation of TLR7-mediated autophagy increases epileptic susceptibility via reduced KIF5A-dependent GABAA receptor transport in a murine model; Authors - Jing Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xuqiao (Verified Customer) (08-31-2020)

It can produce a clear band at 120-130kD which matches it mw, although it also has some uncharacteristic bands below 100 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain

KIF5C Antibody Western Blot validation (1:3000 dilution) in mouse brain (Cat no:25897-1-AP)