KIF26B Polyklonaler Antikörper

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• KD/KO Validated

KIF26B Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 17422-1-AP

Synonyme

KIF26B, kinesin family member 26B, Kinesin like protein KIF26B

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Galerie der Validierungsdaten

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  WB analysis of MDCK using 17422-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17422-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17422-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17422-1-AP (KIF26B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17422-1-AP (KIF26B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17422-1-AP (KIF26B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17422-1-AP (KIF26B Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDCK-Zellen, HEK-293-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 8 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Produktinformation

17422-1-AP bindet in WB, IHC, IF, ELISA KIF26B und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF26B fusion protein Ag11446
• Vollständiger Name: kinesin family member 26B
• Berechnetes Molekulargewicht: 2108aa,224 kDa; 162aa,17 kDa
• Beobachtetes Molekulargewicht: 224-230 kDa, 17-22 kDa
• GenBank-Zugangsnummer: BC042481
• Gene symbol: KIF26B
• Gene ID (NCBI): 55083
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF26B is a member of kinesin-11 family and plays important role in embryogenesis. It is a downstram target of Sall1 and is essential for kidney development. KIF26B is also involved in tumorigenesis.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen ; KD Validated
human	IHC,WB	Biomed Pharmacother; KIF26B promotes cell proliferation and migration through the FGF2/ERK signaling pathway in breast cancer.; Authors - Yan Teng
human,mouse	WB,IF	J Bone Miner Res; KIF26B Silencing Prevents Osseous Transdifferentiation of Progenitor/Stem Cells and Attenuates Ectopic Calcification in a Murine Model.; Authors - Mingming Yan ; KD Validated
human	IF	J Ginseng Res; Ginsenoside Rh2 reduces m6A RNA methylation in cancer via the KIF26B-SRF positive feedback loop; Authors - Chunmei Hu
human	IHC	Cell Cycle; Noncoding RNAs-based high KIF26B expression correlates with poor prognosis and tumor immune infiltration in colon cancer; Authors - Zhihong Liu
mouse	IF	Biochem Biophys Res Commun; Novel mechanistic role of Kif26b in adipogenic differentiation of murine multipotent stromal cells.; Authors - Mingming Yan ; KD Validated