KIF1B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KIF1B Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 15263-1-AP

Synonyme

CMT2, CMT2A, CMT2A1, HMSNII, KIAA0591, KIAA1448, KIF1B, kinesin family member 1B, Kinesin like protein KIF1B, KLP

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15263-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15263-1-AP (KIF1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15263-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15263-1-AP (KIF1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15263-1-AP

  IP Result of anti-KIF1B (IP:15263-1-AP, 5ug; Detection:15263-1-AP 1:700) with HeLa cells lysate 2000ug.

• 

  IHC staining of human liver cancer using 15263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15263-1-AP (KIF1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15263-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15263-1-AP (KIF1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 15263-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15263-1-AP (KIF1B antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Skelettmuskelgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

15263-1-AP bindet in WB, IP, IHC, IF, ELISA KIF1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIF1B fusion protein Ag7263
• Vollständiger Name: kinesin family member 1B
• Berechnetes Molekulargewicht: 204 kDa
• Beobachtetes Molekulargewicht: 199 kDa, 204 kDa
• GenBank-Zugangsnummer: BC001415
• Gene symbol: KIF1B
• Gene ID (NCBI): 23095
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	IP	J Hazard Mater; Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.; Authors - Yiyuan Kang
mouse	IF	Elife; Brucella activates the host RIDD pathway to subvert BLOS1-directed immune defense.; Authors - Kelsey Michelle Wells
mouse	WB	Exp Neurol; Axonal injury mediated by neuronal p75NTR/TRAF6/JNK pathway contributes to cognitive impairment after repetitive mTBI; Authors - Gang Liu
human	WB	Cell Rep; Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells.; Authors - Don-Marc Franchini ; KD Validated
rat	WB	Cell Death Dis; AAV-mediated inhibition of ULK1 promotes axonal regeneration in the central nervous system in vitro and in vivo.; Authors - Vinicius Toledo Ribas

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Maria (Verified Customer) (07-31-2021)

KIF1B in human primary fibroblasts in culture. Reducing and denaturing conditions. 15 ug of total protein. TGX gel 4-15%. Blocking BSA 3%. 1:1000 primary ab O/N 4ºC. 1:5000 secondary HRP antibody 2h RT. Many bands in the blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary fibroblasts

KIF1B Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:15263-1-AP)