VIRMA/KIAA1429 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VIRMA/KIAA1429 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 25712-1-AP

Synonyme

fSAP121, KIAA1429, MSTP054, Protein virilizer homolog, VIRMA, VIRMA/KIAA1429

---

Galerie der Validierungsdaten

• 

  WB analysis of PC-3 using 25712-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25712-1-AP (KIAA1429 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 25712-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25712-1-AP (KIAA1429 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 25712-1-AP

  IP result of anti-VIRMA/KIAA1429 (IP:25712-1-AP, 4ug; Detection:25712-1-AP 1:600) with MCF-7 cells lysate 800 ug.

• 

  IHC staining of human breast cancer using 25712-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25712-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 25712-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, MDA-MB-231-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

25712-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA VIRMA/KIAA1429 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VIRMA/KIAA1429 fusion protein Ag22449
• Vollständiger Name: KIAA1429
• Berechnetes Molekulargewicht: 1812 aa, 202 kDa
• Beobachtetes Molekulargewicht: 200-210 kDa
• GenBank-Zugangsnummer: BC113380
• Gene symbol: KIAA1429
• Gene ID (NCBI): 25962
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as oncogenic factor in breast cancer and liver cancer.

Publikationen

Species	Application	Title
human	WB	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv
human,mouse	WB,IHC,IP	Cell Mol Biol Lett; KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathway; Authors - Xiaomin Chen ; KO Validated
human	WB	Aging (Albany NY); WTAP promotes myocardial ischemia/reperfusion injury by increasing endoplasmic reticulum stress via regulating m6A modification of ATF4 mRNA.; Authors - Jiayi Wang
human	WB	Front Oncol; m6A Methyltransferase METTL14-Mediated Upregulation of Cytidine Deaminase Promoting Gemcitabine Resistance in Pancreatic Cancer.; Authors - Congjun Zhang
human	WB,CoIP	Sci Rep; The m6A-methylase complex recruits TREX and regulates mRNA export.; Authors - Simon Lesbirel ; KD Validated
human	IHC	Med Sci Monit; Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.; Authors - Yingying Zhang