KIAA1217 Polyklonaler Antikörper

KIAA1217 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24880-1-AP

Synonyme

KIAA1217, Sickle tail protein homolog, SKT

---

Galerie der Validierungsdaten

• 

  WB analysis using 24880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24880-1-AP (KIAA1217 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 24880-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 24880-1-AP (KIAA1217 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24880-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24880-1-AP (KIAA1217 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24880-1-AP

  Rat colon lysates were subjected to SDS PAGE followed by western blot with 24880-1-AP (KIAA1217 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maus-Kolongewebe, Ratten-Kolongewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24880-1-AP bindet in WB, ELISA KIAA1217 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIAA1217 fusion protein Ag19367
• Vollständiger Name: KIAA1217
• Berechnetes Molekulargewicht: 1943 aa, 214 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa, 140-160 kDa, 210 kDa
• GenBank-Zugangsnummer: BC018764
• Gene symbol: KIAA1217
• Gene ID (NCBI): 56243
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIAA1217, sickle tail protein homolog, is required for normal development of intervertebral disks. It promotes epithelial-mesenchymal transition and hepatocellular carcinoma metastasis by interacting with and activating STAT3, indicating its potential as a therapeutic target for curbing HCC metastasis (PMID: 35008530). It is also a novel candidate gene associated with isolated and syndromic vertebral malformations (PMID: 32369272). KIAA1217 has 9 alternatively spliced isoforms of 100-110 kDa, 140-160 kDa, 210 kDa.