ATG13 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATG13 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 18258-1-AP

Synonyme

ATG13, Autophagy related protein 13, KIAA0652

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 18258-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18258-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18258-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18258-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 18258-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 18258-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 18258-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BGC-823 using 18258-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 18258-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 18258-1-AP

  IP Result of anti-ATG13 (IP:18258-1-AP, 4ug; Detection:18258-1-AP 1:300) with SH-SY5Y cells lysate 1600ug.

• 

  IHC staining of mouse heart using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18258-1-AP (ATG13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18258-1-AP (ATG13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 18258-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 18258-1-AP (ATG13 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, BGC-823-Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Hirngewebe, Jurkat-Zellen, Maus-Cerebellum-Gewebe, Maushodengewebe, Maus-Thymusgewebe, MCF-7-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

18258-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ATG13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATG13 fusion protein Ag13090
• Vollständiger Name: KIAA0652
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57-63 kDa
• GenBank-Zugangsnummer: BC001331
• Gene symbol: KIAA0652
• Gene ID (NCBI): 9776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

Publikationen

Species	Application	Title
mouse	WB	Cell; Noncanonical autophagy promotes the visual cycle.; Authors - Kim Ji-Young JY ; KD Validated
human	WB	J Clin Invest; p53/microRNA-214/ULK1 axis impairs renal tubular autophagy in diabetic kidney disease.; Authors - Zhengwei Ma
mouse		Autophagy; The ALS-FTD-linked gene product, C9orf72, regulates neuronal morphogenesis via autophagy.; Authors - Wan Yun Ho
human	WB	Oxid Med Cell Longev; Silencing TUFM Inhibits Development of Monocrotaline-Induced Pulmonary Hypertension by Regulating Mitochondrial Autophagy via AMPK/mTOR Signal Pathway; Authors - Ruyuan Wei
mouse	WB	Stem Cell Res Ther; Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.; Authors - Caixia Jin
human	WB	Ecotoxicol Environ Saf; Downregulation of beclin 1 restores arsenite-induced impaired autophagic flux by improving the lysosomal function in the brain.; Authors - Hongmei Zhou