KIAA0513 Polyklonaler Antikörper

KIAA0513 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21478-1-AP

Synonyme

KIAA0513

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 21478-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21478-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21478-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 21478-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 21478-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 21478-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIAA0513 antibody 21478-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hodengewebe, Maus-Cerebellum-Gewebe, Maushodengewebe, Mausnierengewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21478-1-AP bindet in WB, IF,ELISA KIAA0513 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIAA0513 fusion protein Ag15624
• Vollständiger Name: KIAA0513
• Berechnetes Molekulargewicht: 411 aa, 47 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC030280
• Gene symbol: KIAA0513
• Gene ID (NCBI): 9764
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIAA0513 was identified as upregulated in subjects with schizophrenia. The mRNA of KIAA0513 is ubiquitously expressed but is enriched in the brain, particularly in the cerebellum. The interacting protein analysis indicated that KIAA0513 is involved in both synaptic and apoptotic signaling. (PMID: 17010949) Several isoforms of KIAA0513 (47/46/34 kDa) exist due to the alternative splicing. This antibody is expected to recognize all of isoforms of KIAA0513.