MARF1 Polyklonaler Antikörper

MARF1 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 25233-1-AP

Synonyme

MARF1, A 362G6.1, KIAA0430, Limkain b1, LKAP

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 25233-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25233-1-AP (KIAA0430 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25233-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25233-1-AP (KIAA0430 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25233-1-AP

  IP Result of anti-KIAA0430 (IP:25233-1-AP, 5ug; Detection:25233-1-AP 1:300) with HeLa cells lysate 2000ug.

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  IHC staining of human small intestine using 25233-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25233-1-AP (KIAA0430 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 25233-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25233-1-AP (KIAA0430 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 25233-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25233-1-AP (KIAA0430 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 25233-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25233-1-AP (KIAA0430 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HEK-293 using 25233-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 25233-1-AP (KIAA0430 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

25233-1-AP bindet in WB, IP, IHC, IF, ELISA MARF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MARF1 fusion protein Ag18905
• Vollständiger Name: KIAA0430
• Berechnetes Molekulargewicht: 1742 aa, 193 kDa
• Beobachtetes Molekulargewicht: 192 kDa
• GenBank-Zugangsnummer: BC137165
• Gene symbol: KIAA0430
• Gene ID (NCBI): 9665
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARF1, also known as KIAA0430, or LKAP, which is expressed in peroxisome. MARF1 is essential regulator of oogenesis and required for female meiotic progression to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. MARF1 probably acts via some RNA metabolic process and is equivalent to the piRNA system in males, which mediates the repression of transposable elements during meiosis by forming complexes composed of RNAs and governs the methylation and subsequent repression of transposons.

Publikationen

Species	Application	Title
human	WB	Front Immunol; Gradient Boosting Machine Learning Model for Defective Endometrial Receptivity Prediction by Macrophage-Endometrium Interaction Modules.; Authors - Bohan Li