DELE1 Polyklonaler Antikörper

DELE1 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (4)

Kat-Nr. : 21904-1-AP

Synonyme

DELE, DELE1, KIAA0141

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 21904-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21904-1-AP (KIAA0141 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21904-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21904-1-AP (KIAA0141 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

21904-1-AP bindet in WB, ELISA DELE1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DELE1 fusion protein Ag14615
• Vollständiger Name: KIAA0141
• Berechnetes Molekulargewicht: 515 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC007855
• Gene symbol: KIAA0141
• Gene ID (NCBI): 9812
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DELE1, also known as KIAA0141, is a 515 amino acid protein, which localizes in Mitochondrion and is detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon. DELE1 is essential for the induction of death receptor-mediated apoptosis through the regulation of caspase activation. Mitochondrial malfunction needs to be relayed to the cytosol, where an integrated stress response is triggered by the phosphorylation of eukaryotic translation initiation factor 2α(eIF2α) in mammalian cells. eIF2αphosphorylation is mediated by the four eIF2α kinases GCN2, HRI, PERK and PKR, which are activated by diverse types of cellular stress. OMA1, a mitochondrial stress-activated protease; and DELE1, a little-characterized protein that we found was associated with the inner mitochondrial membrane. Mitochondrial stress stimulates OMA1-dependent cleavage of DELE1 and leads to the accumulation of DELE1 in the cytosol, where it interacts with HRI and activates the eIF2α kinase activity of HRI. In addition, DELE1 is required for ATF4 translation downstream of eIF2α phosphorylation.

Publikationen

Species	Application	Title
mouse	WB	Antioxidants (Basel); Increased Mobile Zinc Regulates Retinal Ganglion Cell Survival via Activating Mitochondrial OMA1 and Integrated Stress Response; Authors - Jiahui Tang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Damien (Verified Customer) (09-01-2023)

Unfortunately, in agreement with with Guo et al. Nature 2020, this antibody does not recognize endogenous DELE1.

• Applications: Western Blot
• Cell Tissue Type: HEK293T, Hela cells

FH

Süleyman (Verified Customer) (12-06-2022)

CCCP treatment for 6 hour reduced the DELE1 level as it is published but I did not observe any cleaved DELE1, therefore it is hard to tell whether translation of DELE1 is reduced or cleaved. Some cell line has unspecific bands as well. I used 1:200 which is also a lot (antibody can be used quickly), one can test 1:500 or 1:1000 for WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: HeLa and HeLa Flip In TREX

DELE1 Antibody Western Blot validation (1:200 dilution) in HeLa and HeLa Flip In TREX (Cat no:21904-1-AP)

FH

SITING (Verified Customer) (04-02-2021)

GOOD

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEART

FH

Maria (Verified Customer) (02-10-2021)

DELE1 in human primary fibroblasts. 12% TGX gel. PVDF membrane. Blocking 3% BSA in PBST (0.1% Tween), primary incubation 1:1000 in PBST(0.1% Tween)+ 3% BSA 16h 4ºC. Secondary Goat anti rabbit HRP 1:5000 1h RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Primary Fibroblasts

DELE1 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:21904-1-AP)